abstract：:The heat sensitivity at 42 degrees, 43 degrees and 44 degrees C of various hematopoietic subsets in murine bone marrow (MRA, CFU-S-12, CFU-S-8, CFU-GM, BFU-E and CFU-E) was investigated in order to determine whether there is a relationship between heat sensitivity and the position of cells within the stem cell hierarc...

journal_title：Experimental hematology

authors： Wierenga PK,Konings AW

更新日期：1993-05-01 00:00:00

abstract：:Both stimulatory (CSA) and inhibitory (INH) factors may contribute to hematopoietic regulation, but little is known about how their physiologic balance is maintained. Previously we have shown that antigen-defined fibroblastoid cells cultured from rat lung (ST3-/ST4+) constitutively produce INH, and those derived from ...

journal_title：Experimental hematology

authors： Wang H,Sullivan AK

更新日期：1993-05-01 00:00:00

abstract：:Studies were undertaken to produce monoclonal antibodies directed against the murine receptor for macrophage colony-stimulating factor (M-CSF or CSF-1). Sprague-Dawley rats were injected with lysates prepared from a murine myelomonocytic cell line (RAW cell line) that has high levels of M-CSF receptors. Spleen cells f...

journal_title：Experimental hematology

authors： Shadduck RK,Waheed A,Mangan KF,Rosenfeld CS

更新日期：1993-04-01 00:00:00

abstract：:G-banded metaphase chromosomes prepared from 14 male CBA/Ca mice with histologically confirmed myeloid leukemia (ML) were studied in an effort to identify specific chromosomal changes associated with radiation leukemogenesis. The chromosome studies were undertaken as part of a larger investigation of radiation carcino...

journal_title：Experimental hematology

authors： Rithidech KN,Bond VP,Cronkite EP,Thompson MH

更新日期：1993-03-01 00:00:00

abstract：:We have utilized two well-characterized human leukemia/lymphoma (LL) cell lines, UTMB-460 and CEM, to determine the role of integrin very late antigen-alpha 4 beta 1 (VLA-4) and its ligand vascular cell adhesion molecule-1 (VCAM-1) in the adherence of the LL cells to marrow stromal cells (MSC). Both these LL cell line...

journal_title：Experimental hematology

authors： Juneja HS,Schmalsteig FC,Lee S,Chen J

更新日期：1993-03-01 00:00:00

abstract：:Serum levels of monokines, including macrophage colony-stimulating factor (M-CSF), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 alpha (IL-1 alpha) and IL-1 beta (IL-1 beta), were measured in patients with chronic renal failure in an attempt to clarify the kinetics of these cytokines in the course of renal an...

journal_title：Experimental hematology

authors： Kawano Y,Takaue Y,Motoyoshi K,Minakuchi J,Kawashima S,Saito S,Hirao A,Sato J,Shimizu T,Kuroda Y

更新日期：1993-02-01 00:00:00

abstract：:Patients subjected to prolonged exposure to nitrous oxide (N2O) often develop megaloblastic bone marrow changes. This toxicity is due to the N2O-mediated inactivation of cobalamin-dependent enzymes with resultant perturbations in cell metabolism. The effect of N2O on the behavior of murine colony-forming units-cytokin...

journal_title：Experimental hematology

authors： Warren DJ,Slørdal L

更新日期：1993-02-01 00:00:00

abstract：:The current knowledge concerning the blood supply to hematopoietic bone marrow during increased marrow metabolism is scanty. We have previously shown that an accelerated erythropoiesis in the awake rat is accompanied by a rapid increase in perfusion of the tibial marrow and its bony encasement. We have now measured bl...

journal_title：Experimental hematology

authors： Iversen PO,Nicolaysen G,Benestad HB

更新日期：1993-02-01 00:00:00

abstract：:The effects of the inhibition of the cyclooxygenase and lipoxygenase metabolic pathways of arachidonic acid on the postirradiation recovery of hematopoietic functions in mice were investigated. Nordihydroguaiaretic acid (NDGA), an inhibitor of prostaglandin (PG) and leukotriene (LT) production, was given to animals in...

journal_title：Experimental hematology

authors： Kozubík A,Hofmanová J,Holá J,Netíková J

更新日期：1993-01-01 00:00:00

abstract：:Mammalian hemopoiesis results from a complex interaction between hemopoietic progenitor cells, stromal cells and extracellular matrix components, orchestrated by specific glycoprotein growth factors. Recently, these growth factors have been shown to possess an important function, apart from stimulation of proliferatio...

journal_title：Experimental hematology

authors： Minguell JJ,Hardy CL

更新日期：1993-01-01 00:00:00

abstract：:Human cord blood or bone marrow cells expressing the CD34 surface antigen include a population of pluripotent progenitors. We identified and isolated a subpopulation of cells coexpressing CD34 and c-kit, a transmembrane receptor with tyrosine kinase activity. Novel monoclonal antibodies (16A6, 14A3, 3D6) directed agai...

journal_title：Experimental hematology

authors： Reisbach G,Bartke I,Kempkes B,Kostka G,Ellwart J,Birner A,Thalmeier K,Mailhammer R,Bornkamm GW,Ullrich A

更新日期：1993-01-01 00:00:00

abstract：:We have recently shown that interleukin 6 (IL-6) induces transient expression of the alpha-chain of the interleukin 2 receptor (IL-2R alpha) in the murine leukemia myeloid M1 cell line. Others have reported that IL-6 and interleukin 1 (IL-1) synergistically enhance the expression of IL-2R alpha in T cells. Thus, in th...

journal_title：Experimental hematology

authors： Ruhl S,Schwabe M,Pluznik DH

更新日期：1992-11-01 00:00:00

abstract：:DNA methylation plays an important role in gene regulation. We have analyzed the methylation status of CCGG sites in and around the human proto-oncogene c-myc in blood cells from patients with acute and chronic leukemias and with myelodysplastic syndromes using restriction endonucleases. The 5' region of c-myc was une...

journal_title：Experimental hematology

authors： Tsukamoto N,Morita K,Karasawa M,Omine M

更新日期：1992-10-01 00:00:00

abstract：:High-dose chemotherapy followed by autologous bone marrow transplantation (ABMT) in the treatment of malignancies is often associated with immune deficiency following transplantation, possibly contributing to tumor relapse or fatal infection. Interleukin 2 (IL-2), which enhances major histocompatibility complex (MHC)-...

journal_title：Experimental hematology

authors： Bosly A,Guillaume T,Brice P,Humblet Y,Staquet P,Doyen C,Chatelain B,Franks CR,Gisselbrecht C,Symann M

更新日期：1992-09-01 00:00:00

abstract：:Chronic treatment of mice with transforming growth factor beta 1 (TGF-beta 1) resulted in a dose-dependent inhibition of erythropoiesis. Following 14 daily s.c. injections of 5 or 25 micrograms of TGF-beta 1, a significant degree of anemia was observed. In addition, erythroid progenitor cells were present in reduced n...

journal_title：Experimental hematology

authors： Miller KL,Carlino JA,Ogawa Y,Avis PD,Carroll KG

更新日期：1992-09-01 00:00:00

abstract：:Expression of the main nuclear protooncogenes during terminal megakaryocyte (MK) differentiation is poorly understood. Because previous results have suggested that c-fos and c-jun protooncogenes are expressed in human leukemic cell lines induced to undergo megakaryocytic differentiation, we have analyzed the expressio...

journal_title：Experimental hematology

authors： Mouthon MA,Navarro S,Katz A,Breton-Gorius J,Vainchenker W

更新日期：1992-08-01 00:00:00

abstract：:Our previous studies have shown that a unique glycoprotease from Pasteurella haemolytica specifically cleaves only proteins containing sialylated O-linked glycans. The hematopoietic progenitor cell antigen, CD34, which is heavily glycosylated with both N- and O-linked glycans, is readily cleaved by this protease. In t...

journal_title：Experimental hematology

authors： Sutherland DR,Marsh JC,Davidson J,Baker MA,Keating A,Mellors A

更新日期：1992-06-01 00:00:00

abstract：:An evaluation of the effectiveness of a genetically engineered recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF)/interleukin 3 (IL-3) fusion protein (FP) as a means of delivering cytokine combinations to megakaryocyte (MK) progenitor cells was performed, utilizing a serum-depleted clonal assay syst...

journal_title：Experimental hematology

authors： Bruno E,Briddell RA,Cooper RJ,Brandt JE,Hoffman R

更新日期：1992-05-01 00:00:00

abstract：:Injection of mice with either natural bovine bone-derived or human recombinant transforming growth factor beta 1 (TGF-beta 1) resulted in a significant increase of the macrophage and macrophage-granulocyte-forming capacity of their macrophage colony-stimulating factor (M-CSF)- and granulocyte-macrophage colony-stimula...

journal_title：Experimental hematology

authors： Bursuker I,Neddermann KM,Petty BA,Schacter B,Spitalny GL,Tepper MA,Pasternak RD

更新日期：1992-05-01 00:00:00

abstract：:A human acute myelomonocytic leukemia cell line, KBM-3, was developed to study the pathophysiology of human acute myeloid leukemia. This cell line was characterized by morphology, immunophenotype, Giemsa-banding pattern, in vitro proliferation capacity, and tumorigenicity in nude mice. The KBM-3 cell line was establis...

journal_title：Experimental hematology

authors： Andersson BS,Bergerheim US,Collins VP,Childs C,Beran M,Sen S,Arden K,Pathak S,Siciliano MJ,Ost A

更新日期：1992-03-01 00:00:00

abstract：:To assess the thrombopoietic activity of erythropoietin (Epo) in vivo in mice, we consecutively administered recombinant human Epo (rEpo) i.p. to intact and splenectomized mice for varying time intervals. Recombinant human Epo increased platelet counts in a dose-dependent fashion in a short-term trial for 5 days. An 1...

journal_title：Experimental hematology

authors： Shikama Y,Ishibashi T,Kimura H,Kawaguchi M,Uchida T,Maruyama Y

更新日期：1992-02-01 00:00:00

abstract：:Malignant osteopetrosis is a disorder characterized by a deficiency in osteoclast number or function. In one animal model of osteopetrosis, the op/op mouse, macrophage colony-stimulating factor (M-CSF) is absent, and the administration of M-CSF corrects the defects. We evaluated the serum of 13 patients with malignant...

journal_title：Experimental hematology

authors： Orchard PJ,Dahl N,Aukerman SL,Blazar BR,Key LL Jr

更新日期：1992-01-01 00:00:00

abstract：:Blood samples were examined from 25 children with malignancies during hematopoietic recovery following chemotherapy-induced aplasia and from 9 children undergoing tonsillectomy. The proportion of CD34-positive peripheral blood mononuclear cells (PBMNC) evaluated by flow cytometry was compared with the number of coloni...

journal_title：Experimental hematology

authors： Fritsch G,Emminger W,Buchinger P,Printz D,Gadner H

更新日期：1991-12-01 00:00:00

abstract：:In this report, the effects of adenosine on the promyelocytic cell line HL-60 and on T-lymphocytic clones are compared. According to previous reports, adenosine induces a dose-dependent inhibition of DNA synthesis in T-lymphocytes. Conversely, adenosine dose-dependently enhances DNA synthesis in HL-60 cells, as docume...

journal_title：Experimental hematology

authors： Orrico A,Capecchi PL,De Magistris T,Nuti S,Pasini FL

更新日期：1991-11-01 00:00:00

abstract：:The human cell line CMK spontaneously expresses megakaryocytic characteristics and can be induced to differentiate into mature megakaryocytes after exposure to 12-O-tetradecanoylphorbol-13-acetate (TPA). In comparison with CMK, we have examined the characteristics of a subclone, designated as CMK11-5, that is morpholo...

journal_title：Experimental hematology

authors： Adachi M,Ryo R,Sato T,Yamaguchi N

更新日期：1991-10-01 00:00:00

abstract：:Initiation of DNA synthesis by recombinant colony-stimulating factors (CSFs) was assessed in normal human marrow blast cells isolated by expression of CD34 antigen (tritiated thymidine incorporation). Continuous exposure to CSF was required. A mild increase in DNA synthesis was initiated by granulocyte CSF (G-CSF; gre...

journal_title：Experimental hematology

authors： Strauss LC,Welsh SB,Civin CI

更新日期：1991-09-01 00:00:00

abstract：:Recently we have described a CD4+ human T-cell hybridoma Ft.F3 (ATCC HB 9713). This hybridoma produces two proteins having molecular weights of 29 kd (P29) and 14.7 kd (P14.7) that function as activators of human monocyte tumor cytotoxicity and interleukin 1 (IL-1) synthesis (macrophage cytotoxicity-inducing factors, ...

journal_title：Experimental hematology

authors： Jones CM,Prince CA,Williams JS

更新日期：1991-08-01 00:00:00

abstract：:Mice with the nu mutation have been shown to have a reduced incidence of spleen colony-forming units (CFU-S), with a higher variability between individual mice compared to +/+ mice. The presence of these defects also in nu/+ mice indicates that this mutation is a codominant trait and that the hematopoietic changes may...

journal_title：Experimental hematology

authors： Necas E,Znojil V,Holub M

更新日期：1991-08-01 00:00:00

abstract：:We have previously shown that CD33- CD34+ human marrow cells are capable of giving rise to colony-forming cells (CFC) in long-term marrow culture (LTMC) but are mainly depleted of progenitors that directly form colonies in semi-solid media. In contrast, the CD33+ CD34+ population contains most of the CFC but not the p...

journal_title：Experimental hematology

authors： Bernstein ID,Leary AG,Andrews RG,Ogawa M

更新日期：1991-08-01 00:00:00

abstract：:The immunostimulant poly(A)-poly(U) induces a rapid enhancement of circulating colony-stimulating activity (CSA) in normal mice, culminating 2 h after i.v. injection. A dose of 200 micrograms per mouse is sufficient for a maximal effect. The colonies formed in response to sera from poly(A)-poly(U)-injected mice are ma...

journal_title：Experimental hematology

authors： Dy M,Arnould A,Schneider E

更新日期：1991-05-01 00:00:00

abstract：:Based on previous observations that granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes granulocyte recovery following chemotherapy, we evaluated the effect of recombinant human GM-CSF on hematopoietic progenitors and clinical outcome in six patients with delayed engraftment (greater than 55 days) after...

journal_title：Experimental hematology

authors： Brandwein JM,Nayar R,Baker MA,Sutton DM,Scott JG,Sutcliffe SB,Keating A

更新日期：1991-03-01 00:00:00

abstract：:An improved negative immunomagnetic selection strategy has been devised for the enrichment of primitive hemopoietic cells using the high proliferative potential colony-forming cell (HPP-CFC) assay as an index of stem cell purification. Immunomagnetic selection was carried out using goat anti-rat conjugated M-450 Dynab...

journal_title：Experimental hematology

authors： Bertoncello I,Bradley TR,Watt SM

更新日期：1991-02-01 00:00:00

abstract：:Pathogenesis of cytomegalovirus (CMV)-induced myelosuppression is not clearly understood and could be related to a direct toxic effect on the marrow progenitors and/or an alteration of the marrow environment. Myeloid progenitors (granulocyte-macrophage colony-forming units, CFU-GM) were not affected by incubation with...

journal_title：Experimental hematology

authors： Bron D,Lagneaux L,Delforge A,Otte C,Snoeck R,Stryckmans P

更新日期：1991-02-01 00:00:00

abstract：:Different human hematopoietic cell lines were analyzed for the presence of interleukin 6 (IL-6) and IL-6 receptor (IL-6-R). Both IL-6 mRNA and secreted active IL-6 protein were detectable in untreated cell lines with erythroid or megakaryoblastic features (K562, HEL, KU 812, MEG-01, and Dami), but they were not expres...

journal_title：Experimental hematology

authors： Navarro S,Mitjavila MT,Katz A,Doly J,Vainchenker W

更新日期：1991-01-01 00:00:00

abstract：:In a dose escalation study we tested the feasibility and tolerance of high-dose recombinant human erythropoietin (r-HuEPO) therapy in four patients with ineffective erythropoiesis due to myelodysplastic syndromes (MDS) or paroxysmal nocturnal hemoglobinuria (PNH). Recombinant human EPO was administered i.v. with an in...

journal_title：Experimental hematology

authors： Stebler C,Tichelli A,Dazzi H,Gratwohl A,Nissen C,Speck B

更新日期：1990-12-01 00:00:00

abstract：:We present the first report of a T-helper cell line, HUT 102, constitutively producing both granulocyte-macrophage colony-stimulating factor (GM-CSF) and monocytic colony-stimulating factor (M-CSF), as detected by sensitive enzyme-linked immunosorbent assays and Northern blot analysis. However, neither amplification n...

journal_title：Experimental hematology

authors： Kawasaki C,Okamura S,Omori F,Shimoda K,Hayashi S,Kondo S,Yamada M,Niho Y

更新日期：1990-11-01 00:00:00

abstract：:In vitro colony formation by erythroid progenitors from the bone marrow was studied in 42 patients with a myelodysplastic syndrome using both a standard assay for growing erythroid colonies and an assay designed for growing megakaryocyte colonies. In the standard assay 5 patients had normal numbers of erythroid burst-...

journal_title：Experimental hematology

authors： Juvonen E,Ruutu T

更新日期：1990-11-01 00:00:00

abstract：:A novel polypeptide with a molecular weight of 17 kd (17k protein) was identified in bone marrow cell cultures. The synthesis of 17k protein is elevated in cell cultures maintained under Dexter conditions, which support myelopoiesis. The predominance of macrophages in the stromal layer of these cultures and the observ...

journal_title：Experimental hematology

authors： Ruland LJ 3rd,Quesenberry PJ,Balian G

更新日期：1990-10-01 00:00:00

abstract：:We have previously reported that granulocyte-macrophage colony-stimulating factor (GM-CSF) given after the administration of 5-fluorouracil (5-FU) results in augmented hematopoietic recovery as evidenced by increased white blood cell and neutrophil counts. Mice receiving GM-CSF following 5-FU administration were obser...

journal_title：Experimental hematology

authors： O'Reilly M,Gamelli RL

更新日期：1990-10-01 00:00:00

abstract：:Recombinant human interleukin 6 (rhIL-6) was administered s.c. every 12 h at a daily dose of 10 micrograms/kg body weight to normal healthy mice. After 4 days the numbers of progenitor cells (erythroid burst-forming units, BFU-E and granulocyte-macrophage colony-forming cells, GM-CFC) were significantly increased (p l...

journal_title：Experimental hematology

authors： Pojda Z,Tsuboi A

更新日期：1990-10-01 00:00:00